Actin filaments (cyan) under a microfluidic flow being severed upon exposure to a flow containing fluorescently labelled cofilin (yellow). Left : Dual color movie showing both actin and cofilin;
Right: Cofilin only.
Actin filaments nucleated from surface-anchored formins (mDia1) elongating in a microfluidic flow containing unlabelled actin monomers and profilin.
Cofilin-decorate actin filament (red) depolymerizing by single molecules of Srv2/CAP (green, asterisk) transiently processively tracking the pointed end of the filament.